Microflow Verbindungen in verschiedenen Formen und Anwendungen

MICRO TIGHT® FITTINGS AND SLEEVES For Coned ports - For Connecting Capillary Tubing / Made from PEEK™ Polymer
Upchurch Scientific® MicroTight One-Piece Fittings are designed for use with some of our NanoPorts™ and our MicroTight Unions, Adapters and Inline MicroFilters. Specifically made for 360µm OD tubing, 1/32" OD tubing and our MicroTight Tubing Sleeves (discussed below), these fittings make superior fingertight connections with capillary tubing. MicroTight Fittings withstand temperatures up to 125°C.
The MicroTight family also includes a female nut matched with one of five dedicated ferrules for connecting specific tubing ODs:
F-172   70-520µm OD (with MicroTight Tubing Sleeves)
F-152/F-152BLK 360µm OD F-1 12 1/32" OD F-132   1/16" OD		 The OD of each MicroTight Tubing Sleeve is .025", with a variety of IDs available to accommodate 70-520µm OD capillary tubing. Choose the tubing sleeve appropriate for the outer diameter of your capillary tubing by referring to the product listings below. Our MicroTight Connector Kit contains all you need to make most basic capillary connections, at a substantial savings over individually purchased components.
Each 1.25" long sleeve will hold tubing to 4,000 psi (276 bar) and can be used at temperatures up to 125°C. MicroTight Fittings that do not require sleeves are rated to 5,000 psi (345 bar).
Use our P-277 Extender Tool to tighten standard head 6-32 fittings in hard-toreach places. Tighten headless 6-32 fittings with our N-290 Tool.

MICROTIGHT FITTINGS
Artikelnummer Beschreibung Bild Menge  
F-112 MicroFerrule for 1/32" OD tubing, Natural PEEK ea.  
F-124H Headless Fittings for 360µm OD tubing, 6-32, Blue PEEK ea.  
F-124S Std. Head Fittings for 360µm OD tubing, 6-32, Blue PEEK ea.  
F-125 Std. Head Fittings for MicroTight Sleeves, 6-32, Natural PEEK ea.  
F-125H Headless Fittings for MiaoTight Sleeves, 6-32, Natural PEEK ea.  
F-126H Headless Fittings for 1/32" OD tubing, 6-32, Red PEEK ea. Mindestabnahme 25 Stück  
F-126Sx Std. Head Fittings for 1/32" OD tubing, 6-32, Red PEEK 10-pk  
F-132 MicroFerrule for 1/16" OD tubing, Natural PEEK ea.  
F-152 MicroFerrule for 360µm OD tubing, Natural PEEK ea.  
F-152BLK MicroFerrule for 360µm OD tubing, Black PEEK ------------------- ea.  
F-172 MicroFerrule for.025" OD tubing, Black PEEK ea.  
N-290 Extender Tool for Headless 6-32 Fittings ------------------- ea.  
P-116 MicroFerrule Plug, Black PEEK (1) ea.  
P-277 Extender Tool for Standard Head 6-32 Fittings ------------------- ea.  
P-416 Female Nut, 5/16-24 internal threads, Natural PEEK (2) ea.  
P-416BLK Female Nut, 5/16-24 internal threads, Black PEEK (2) ea.  
P-416G Female Nut, 5/16-24 internal threads, Green PEEK (2) ea.  
P-555 Std. Head Plug, 6-32, Natural PEEK (3) ea.  
1 Can only be used with products designed to use the F-152 and F-172 Ferrule. 2 Can be used with F-112, F-132, F-152, F-1528LH, F-172 or P-116.      3 Can be used to plug any of our 6-32 threaded ports.

MICROTIGHT PEEK TUBING SLEEVES AND KITS, .025"OD
Artikelnummer ID OD Size Color Menge  
F-180 125µm (.005") 70 - 110µm Red ea.  
F-181 180µm (.007") 125 - 165µm  Yellow ea.  
F-182 230µm (.009") 175 - 215µm Natural ea.  
F-183 280µm (.011") 225 - 265µm  Blue ea.  
F-184 330µm (.013") 275 - 315µm Orange ea.  
F-185 395µm (.0155") 340 - 380µm Green ea.  
F-186 455µm (.018") 400 - 440µm Black ea.  
F-187 535µm (.021") 480 - 520µm Natural ea.  
1328 MicroTight Tubing Sleeve Kit contains (6) each of the sleeve sizes listed above  
1356 MicroTight Connector Kit Kit contains: a 10-pack of each MicroTght Tubing Sleeve (F-180 - F-187); (2) P-770 MicroTight Adapters; and (2) MicroTight P-720 Unions  
 
CONDUCTIVE MICROTIGHT UNION
 MICROTIGHT UNIONS
The Conductive MicroTight Union now available from Upchurch Scientific® provides an excellent opportunity to introduce voltage into an electrospray or capillary electrophoresis system. With an extremely low internal volume of 16nL, the union can be placed inline with 360µm OD capillary tubing. Mount and apply voltage to this union using our Insulating Mounting Bracket. Connect two pieces of capillary tubing with these Upchurch Scientific® PEEK™ MicroTight Unions. Choose from our selection of MicroTight Tubing Sleeves to use with the true zero dead volume (ZDV) P-720 Union. The P-771 True ZDV Union allows direct connection of 1/32" OD tubing without sleeves. The True ZDV Unions allow two pieces of tubing to connect directly to each other - using the included gauge plug to ensure proper alignment (also applies to MicroTight Adapters). Our P-772 Union direct connects popular 360µm OD capillary tubing without sleeves, adding only 5nL of swept volume with its .006" (150µm) thru-hole.

Artikelnummer Beschreibung Threads Thruhole Swept Volume Bild  
MICROTIGHT UNIONS
M-572 Conductive Union, PEEK /SST 5/16-24 to 5/16-24 .011" (279µm) 16 nL  
Includes (2) F-152, (2) P-416BLK Fittings and (1) MN-128NF Capsule Union
P-720 True ZDV Union, PEEK 6-32 to 6-32 N/A N/A  
Includes (2) F-152 and (2) P-416BLK Fittings
P-771 True ZDV Union, PEEK 6-32 to 6-32 N/A N/A  
Includes (2) F-126S Fittings and (1) P-553 Gauge Plug
P-772 Union, PEEK 5/16-24 to 5/16-24 .006" (150µm) 5nL  
Includes (2) F-152 and (2) P-416BLK Fittings
REPLACEMENT GAUGE PLUGS
(To achieve true ZDV connections with our P-720 and P-771 Unions)
P-553 Gauge Plug, Delrin® 6-32 None None -------------------  
P-554 Gauge Plug, Delrin® 10-32 None None -------------------  
SST = Stainless Steel

MICROTIGHT® ADAPTERS
Create a true zero dead volume (ZDV) connection between 1/16" OD tubing and capillary tubing with these Upchurch Scientific® PEEK™ MicroTight Adapters. P-770 connects 1/16" OD tubing to a variety of capillary tubing sizes using MicroTight Tubing Sleeves. Our P-881 and P-882 adapters allow connection of 1/16" OD tubing directly to 1/32" OD and 360µm tubing, respectively. !! Please note: use only the 6-32 threaded fitting supplied with each adapter they are not interchageable !!

LUER-TO-MICROTIGHT ADAPTER
The new Upchurch Scientific Luer-to-Microtight Adapter is ideal for infusing sample into lab-on-a-chip devices. This product is made entirely
of biocompatible PEEK
polymer and introduces no dead volume to the flow path. Use it to directly connect a luer-tip syringe or other standard male luer to 360µm OD capillary tubing without tubing sleeves (see photo). MicroTight Fittings are included.

Artikelnummer Beschreibung Threads Thruhole  
MICROTIGHT ADAPTERS
P-770 True ZDV Adapter, PEEK   Includes(1) F-120 Fitting, (1) F-125 Fitting and (1) P-554 Gauge Plug  10-32 to 6-32 N/A  
P-881 True ZDV Adapter, PEEK   Includes (1) F-1 20R Fitting, (1) F-1 26S Fitting and (1) P-554 Gauge Plug 10-32 to 6-32 N/A  
P-882 True ZDV Adapter, PEEK   Includes (1) F-1 20B Fitting, (1) F-1 24S Fitting and (1) P-554 Gauge Plug
 10-32 to 6-32 N/A  
REPLACEMENT GAUGE PLUGS
(To achieve true ZDV connections with our P-770, P-881 and P-882 Adapters)
P-553 Gauge Plug,Delrin® 6-32 None  
P-554 Gauge Plug, Delrin® 10-32 None  
LUER-TO-MICROTIGHT ADAPTER
P-662 Luer-to-MicroTight Adapter Includes (1) F-152 Ferrule and (1) P-416 Nut .006" (150µm)  

MICRO TEE, CROSS AND ELBOW - Direct Connect 1/16", 1/32", 360µm OD Tubing, plus other Capillary Tubing / Low Swept Volume
Use Upchurch Scientific® MicroTees, MicroCrosses or MicroElbow to join capillary tubing. All of these products are made entirely of PEEK and have .006" (150µm) thru-holes, with resulting swept volumes ranging from 20 to 81nL.
Several of these products are designed to connect capillary tubing of various ODs, using our MicroTigh® Tubing Sleeves. Versions for direct connecting 1/16", 1/32" and 360µm OD tubing are also available. In addition, the MicroElbow and one version of the MicroTee come with convenient predrilled .13" (3.3mm) mounting holes.

MICROTEE, MICROCROSS AND MICROELBOW
Artikelnummer Beschreibung Swept Volume  
P-775 MicroTee for MicroTight Sleeves
Includes (3) F-172 and (3) P-416 Fittings		 29nL  
P-777 MicroCross for MicroTight Sleeves
Includes (4) F- 172 and (4) P-416 Fittings		 38nL  
P-874 MicroElbow with Mounting Hole, for MicroTight Sleeves
Includes (2) F-172 and (2) P-416 Fittings		 20nL  
P-875 MicroTee with Mounting Hole, for MicroTight Sleeves
Includes (3) F-172 and (3) P-416 Fittings		 29nL  
P-885 MicroTee for 1/32" OD tubing
Includes (3) F-1 12 and (3) P-416 Fittings		 29nL  
P-887 MicroCross for 1/32" OD tubing
Includes (4) F-1 12 and (4) P-416 Fittings		 38nL  
P-888 MicroTee for 360µm OD tubing
Includes (3) F-152 and (3) P-416BLK Fittings		 29nL  
P-889 MicroCross for 360µm OD tubing
Includes (4) F-152 and (4) P-416BLK Fittings		 38nL  
P-890 MicroTeetor 1/16" OD tubing
Includes (3) F-132 and (3) P-416 Fittings		 58nL  
P-891 MicroCross for 1/16"ODtubing
Includes (4) F-132 and (4) P-416 Fittings		 81nL  

NANOPORTTM ASSEMBLIES
-For Lab-on-a-Chip Applications
Upchurch Scientific® NanoPort Assemblies are the first commercially available products to provide consistent fluid connections for chip-based analyses. These patent-pending products bond easily to chip surfaces with the provided Preformed Adhesive Rings or with alternative Epoxy Adhesive. Once attached, NanoPort connections withstand pressures to 1,500 psi (103 bar). NanoPorts will adhere to silicon, quartz, glass and polymers and will handle 1.5 - 6.0 in.-Ibs. (0.17 N-m to 0.68 N-m) of torque, depending on the substrate (typical fittings require only .25 - .50 in-lbs. tighten). All NanoPort components are made of inert, biocompatible PEEK™ polymer (nuts and ports) and Perlast® perfluoroelastomer (ferrules and gaskets). Their unique design also prevents adhesive contamination of the fluid path. And NanoPort connections add no additional volume to the fluid path, eliminating dead volume traditionally associated with chip-based fluid connections.
Choose from several options to direct-connect 360µm, 1/32" and 1/16" OD tubing. Or use our N-125H or N-125S with a MicroTightTM Tubing Sleeve to connect capillary tubing with ODs of 70 - 520µm. Our NanoPort Reservoir Assembly is designed for open well applications, such as CE. To select the appropriate NanoPort assembly you will need to consider: 1. Size of tubing you are connecting
2. Dimensions of the chip hole
3. Fitting style (one-piece or two-piece fittings)
4. Nut head style (standard or headless nut)
Please Note: each NanoPort Assembly includes a fitting (one- or two-piece), a NanoPort, gasket, a 2-pack of preformed adhesive rings, and a clamp for holding the port in place while the adhesive cures.
Custom NanoPort component configurations are available to Original Equipment Manufacturers (OEMs), including multiport manifolds, alternative thermoplastic materials, special component sizing and specific reservoir volumes. Please contact us for more information.

Application Note
      NanoPort reservoir Applications: -sample reservoir / Open wells for capillary alectrophoresis / Syringe injection or flushing/priming, using our P-604 Luer Adapter and luer syringe       NanoPort Adhesive Cure requirements: - Preformed Adhesive Rings (included with each order) / Alternative Epoxy Adhesive (N-008):  Room temperature cure for 24 hours, or cure at 120°F (49°C) for one hour / Please contact us regarding adherence to specific polymer substrates and other adhesive options
Cure Temperature Cure Time
121°F/121°C
 95 Minutes
275°F/135°C
 42 Minutes
300°F/149°C
 20 Minutes
325°F/163°C
 12 Minutes

Artikelnummer Nut Ferrule For Chip Hole (dia.x depth) For Tubing OD Bild  
NANOPORT ASSEMBLIES
6-32 Flat-Bottom NanoPort Assemblies (1)
N-121H F-123H Hdls. N-123-04 1mm x 1mm 360µm  
N-121S F-123S Std. N-123-04 1mm x 1mm 360µm  
N-123H F-123H Hdls. N-123-03 1mm dia. or less 360µm  
N-123S F-123S Std. N-123-03 1mm dia. or less 360µm  
6-32 Coned NanoPort Assemblies (1)
N-124H F-124H Hdls. None Up to 1/16" 360µm  
N-124S F-124S Std. None Up to 1/16" 360µm  
N-125H F-125H Hdls. None Up to 1/16" 70-520µm (3)  
N-125S F-125 Std. None Up to 1/16" 70-520µm (3)  
N-126H F-126H Hdls. None Up to 1/16" 1/32"  
N-126S F-126S Std. None Up to 1/16" 1/32"  
10-32 Flat-Bottom NanoPort Assemblies (1)
N-129H F-122H N-123-04 1mm x 1mm 360µm  
10-32 Coned NanoPort Assembly (1)
N-333 F-333N F-142N Up to 1/16" 1/16"  
NanoPort Reservoir Assembly (2)
N-131 80µL Reservoir with Insert  
Abbreviation Definitons: FB = Flat-Bottom; n/a = Not Applicable dia. = Diameter; Std. = Standart; Hdls. = Headless (1) Exept as noted, each Nanoport Assembly includes (1) nut; (1) ferrule; (1) NanoPort; (1) gasket; (1) N-100-01 Preformed Adhesive Rings (2-pack); and (1) N-006 Clamp (2) Includes (1) reservoir NanoPort; (1) Insert; (1) N-123-02 Gasket; (1) N-100-01 Preformed adhesive Rings (2-pack); and 81) N-006 Clamp (3) desinged to use our microtight tubing Sleeves to connect tubing OD sizes from 70-520µm

NANOPORT REPLACEMENT PARTS
Artikelnummer Product For Port For Chip Hole (dia. x depth) For tubing OD Menge  
Fittings
F-123H Headless Nut 6-32 FB n/a 360µm ea.  
F-123S Std. Head Nuts 6-32 FB n/a 360µm ea. Mindestabnahme 25 Stück  
F-124H Headless Nuts 6-32 Coned n/a 360µm ea.  
F-124S Std. Head Nuts 6-32 Coned n/a 360µm ea.  
F-125H Headless Nuts 6-32 Coned n/a 70-520µm (3) ea.  
F-125 Std. Head Nuts 6-32 Coned n/a 70-520µm (3) ea.  
F-126H Headless nuts 6-32 Coned n/a 1/32" ea. Mindestabnahme 25 Stück  
F-126Sx Std. Head Nuts 6-32 Coned n/a 1/32" 10-pk  
F-333Nx Hdls. Nuts/Ferrules 10-32 Coned Up to 1/16" 1/16" 10-pk  
F-142Nx Ferrules 10-32 Coned Up to 1/16" 1/16" 10-pk  
N-123-03 Ferrules 6-32 FB 1mm dia. or less 360µm ea.  
N-123-04x Ferrules 6-32, 10-32 FB 1mm dia. x 1mm 360µm 10-pk  
Gaskets
N-123-02 Gasket, For all assemblies except 6-32 Coned Assemblies    ea.  
N-124-02 Gasket, For 6-32 Coned Assemblies ea.  
Adhesives and Clamp
N-006 Clamp ea.  
N-100-01 Preformed Adhesive Rings 2-pk  
Abbreviation Definitions: FB = Flat-Bottom; n/a = Not Applicable dia. = Diameter; Std. = Standart; Hdls. = Headless (3) desinged to use our microtight tubing Sleeves to connect tubing OD sizes from 70-520µm

EXTENDER TOOLS
Upchurch Scientific® offers Extender Tools to make it easier to tighten fittings which would otherwise be too close for adequate finger tightening. The special design of these
tools allows tightening without damaging a nut's knurled head. Use our P-277 Extender Tool to tighten standard head 6-32 fittings. The N-290 Extender Tool is designed for tightening headless 6-32 and 6-40 fittings. Tighten headless 10-32 fittings with our P-297 Extender Tool.

EXTENDER TOOLS
Artikelnummer Beschreibung Menge  
N-290 For Headless 6-32 (and 6-40) Fittings    ea.  
P-277 For Standard Head 6-32 Fittings    ea.  
P-297 For Headless 10-32 Fittings    ea.  

MINI MICROFILTERS
- Total Volume as Low as 10nL! / Conductive Version for CEC and Mass Spectrometry Applications
Upchurch Scientific® Mini MicroFilter Assemblies filter effectively with internal volumes low enough to ensure reliable chromatographic results - even at nanoliter flow rates! They achieve this using 1 µm and 2µm porosity replaceable filter capsules - some versions with a thin, stainless steel micro screen (.25mm thick x 1 mm diameter) and others with a miniscule sintered stainless steel (SST) or titanium (Ti) frit disc (.25mm thick x.5mm diameter). At these sizes, the filtration micro-screen and discs are just a little bigger than the diameter of the period at the end of this sentence. Internal volumes of these encapsulated filters are as low as 85nL with the micro-screen and 10nL to 22nL with the frit disc option!
The micro-screen filter capsules have versions designed for connecting capillary tubing (70-520µm OD) in combination with our MicroTight® Tubing Sleeves, or for direct connecting 360µm and 1/32" (790µm) OD tubing. The frit-disc filter capsules (NanoFilters) are only available for versions that directly connect 360µm and 1/32" OD tubing. The fittings supplied with each MicroFilter are not interchangeable.
Apply voltage to the stainless steel filter holder body of the Conductive Mini MicroFilter for applications such as mass spectrometry and CEC analysis. The voltage is conducted through to the stainless steel portion of the 1 µm NanoFilter Capsule and on to the fluid stream. Try our Insulating Mounting Bracket to apply voltage easily.
Please Note: the MiniFilters that require MicroTight Tubing Sleeves to connect tubing are pressure rated to 4,000 psi (276 bar). Those designed to directconnect tubing without sleeves are pressure rated to 5,000 psi (345 bar).

MINI MICROFILTERS COMPONENTS
Mini MicroFilter Assembly Filter Capsule Porsity Frit Material Fittings For use with tubing OD Size Volume
M-530 M-122 2µm SST Screen F-172, P-416 70-520µm 85nL
M-531 M-121 1µm SST Screen F-172, P-416 70-520µm 85nL
M-532 M-124 2µm SST Screen F-152, P-416BLK 360µm 85nL
M-533 M-123 1µm SST Screen F-152, P-416BLK 360µm 85nL
M-534 M-128 1µm SST Frit F-152, P-416BLK 360µm 10nL
M-537 M-125 1µm SSt Frit F-152, P-416BLK 360µm 10nL
M-538 M-126 1µm Ti Frit F-152, P-416BLK 360µm 10nL
M-542 M-132 2µm SST Screen F-112,P-416 1/32" (790µm) 97nL
M-543 M-131 1µm SST Screen F-112, P-416 1/32" (790µm) 97nL
M-547 M-133 1µm SST Frit F-112, P-416 1/32" (790µm) 22nL
M-548 M-134 1µm Ti Frit F-112, P-416 1/32" (790µm) 22nL
Filter Capsule Color Identtification

MINI MICROFILTER ASSEMBLY
Artikelnummer Beschreibung  
For use with MicroTight Tubing Sleeves for capillary tubing
M-530 2µm Mini MicroFilter Assembly, SST Screen
Includes (5) 2µm M-122 Filter Capsules, (2) F-172 and (2) P-416 Fittings		  
M-531 1pm Mini MicroFilter Assembly, SST Screen  Includes (5) 1 pm M-121 Filter Capsules, (2)F-172 and (2) P-416 Fittings  
For direct-connect use of 360µm OD capillary tubing
M-532 2µm Mini MicroFilter Assembly, SST Screen
Includes (5) 2µm M-124 Filter Capsules, (2) F-152 and (2) P-416BLK Fittings		  
M-533 1µm Mini MicroFilter Assembly, SST Screen
Includes (5) 1 µm M-123 Filter Capsules, (2) F-152 and (2) P-416BLK fittings		  
M-537 1µm Mini MicroFiIterAssembly, SST Frit Includes (5) 1 µm M-125 NanoFilter" Capsules, (2) F-152 and (2) P-416BLK Fittings  
M-538 1µm Mini MiuoFilter Assembly, Ti Frit
Includes (5) 1   M-126 NanoFilter Capsules, (2) F-152 and (2) P-416BLK Fittings		  
For direct-connect use of 1/32" OD tubing
M-542 2µm Mini MiuoFilter Assembly, SST Screen
Includes (5) 2µm M-1 32 Filter Capsules, (2) F-112 and (2) P-416 Fittings		  
M-543 1 µm Mini MiuoFilter Assembly, SST Screen
Includes (5) 1 µm M-131 Filter Capsules, (2) F-112 and (2) P-416 Fittings		  
M-547 1  µm Mini MiuoFilter Assembly, SST Frit Includes (5) 1 µm M-1 33 NanoFllter Capsules, (2) F-112 and (2) P-416 Fittings  
M-548 1 µm Mini MiaoFilter Assembly, l i Frit Includes (5) 1 µm M-134 NanoFilter Capsules, (2) F-112 and (2) P-416 Fittings  
Conductive, for direct-connect use with 360µm OD tubing
M-534 1 µm Conductive Mini MicroFiIterAssembly, SST Frit
Includes (5) 1 µm M-1 28 NanoFilter Capsules, (2) F- 152 and (2) P-416BLK Fittings		  

REPLACEMENT MINI MICROFILTER CAPSULES
Artikelnummer Beschreibung Menge  
For use with M-530 and M-531
M-121 1 µm Filter Capsule, PEEK with SST filter screen 2-pk  
M-122 2µm Filter Capsule, PEEK with SST filter screen 2-pk  
For use with M-532 and M-533
M-123 1 µm Filter Capsule, PEEK with SST filter screen 2-pk  
M-124 2µm Filter Capsule, PEEK with SST filter screen 2-pk  
For use with M-537 and M-538
M-125 1µm NanoFilter Capsule, PEEK with SST frit 2-pk  
M-126 1µm NanoFilter Capsule, PEEK with Ti frit 2-pk  
For use with M-547 and m-548
M-133 1µm NanoFilter Capsule, PEEK with SST frit 2-pk  
M-134 1µm NanoFilter Capsule, PEEK with TI frit 2-pk  
Conductive, For use with M-534
M-128 1µm Conductive NanoFilter Capsule, SST/PEEK with SST Frit 2-pk  

INLINE MICROFILTERS
- 100% Biocompatible PEEK™ Polymer Option Available / As low as 109nL Void Volume
Upchurch Scientific® Inline MicroFilters protect your column from particles originating in the mobile phase or sample, or from pump seal and sample injection valve wear. These filters have a .006" (150µm) thru-hole, and come complete with five PEEK polymer end fittings, each containing a filtering frit. Choose the M-520 with a 0.5µm 100% PEEK frit or the M-135 1.0µm stainless steel filtering screen version, with total theoretical void volumes of 240nL and 109nL, respectively (please see the Mini MicroFilter for more information regarding the micro screen incorporated into the M-135). Also included are two F-125 Micro Fingertight Fittings, designed to work with our MicroTight® Tubing Sleeves and capillary 70-520µm OD tubing. Replacement PEEK Filter End Fittings are available in 10 packs. These filters are pressure rated to 4,000 psi (276 bar).

PRECOLUMN MICROFILTERS
- Direct Connects to Most Columns with 10-32 Threads / total Void Volume of 0.5µL
These Precolumn MicroFilters have 10-32 standard male threads for direct connecting into your microbore or analytical column. Total theoretical void volume is only 0.5µL (includes frit volume) and the PEEK tubing used in the assembly of these units has a .005" (125µm) ID, virtually eliminating any mixing. Two types of filter assemblies are available: one for standard 1/16" OD tubing, the other for 70-520µm OD capillary tubing using our MicroTight Tubing Sleeves. Each Upchurch Scientific MicroFilter Assembly comes complete with 0.5µm replacement stainless steel or PEEK frits, and is pressure rated to 4,000 psi (276 bar).

Artikelnummer Beschreibung Menge  
INLINE MICROFILTERS
For use with MicroTight Tubing Sleeves for capillary tubing
M-520 0.5µm Inline MicroFilter Assembly, PEEK Frit
Includes (5) 0.5µm M-120 MicroFilter End Fittings and (2) F-125 Fittings		  
M-135 1.0µm Inline MicroFilter Assembly, SST Screen
Includes (5) 1.O
µ
m M-130 MicroFilter End Fittings and (2) F-125 Fittings
  
REPLACEMENT INLINE MICROFILTER END-FITTINGS
M-120x End-Fittings, Black, with 0.5µm PEEK Frit 10-pk  
PRECOLUMN MICROFILTER ASSEMBLES
For use with MicroTight Tubing Sleeves for capillary tubing
M-500 O.5µm Precolumn MicroFilter Assembly, SST Frit
Includes (5) 0.5µm C-425 Frits, (1) F172 and (1) P-416 Fitting		  
M-510 O.5µm Precolumn MicroFilter Assembly, PEEK Frit
Includes (5) 0.5µm A-735 Fete, (1) F-172 Ferrule and (1) P-416 Fitting		  
For use with 1/16" OD tubing
M-550 0.5µm Precolumn MicroFilter Assembly, SST Frit
Includes (5) 0.5µm C-425 Frits, (1) F-132 Ferrule and (1) P-416 Hitting		  
M-560 0.5µm Precolumn MicroFilter Assembly, PEEK Frit
Includes (5) 0.5µm A-735 Fries, (1) F-132 Ferrule and (1) P-416 Fitting		  
Replacement Precolumn MicroFilter Frits
Artikelnummer Beschreibung Popsity Menge  
A-735x PEEK Frits,.045"x.031"x.192" 0.5µm 10-pk  
C-420 SST Frits,.038" x.025" x.192" 2.0µm ea.  
C-425 SST Frits,.038" x.028" x.192" 0.5µm ea. Mindestabnahme 25 Stück  

CAPILLARY SAMPLE TRAP COLUMNS
- Optimized for Protein and Peptide Separation/Purification / Nonconductive and Conductive Options / Packed and Unpacked Columns
Upchurch Scientific® Capillary Sample Trap Columns are ideal for separating and concentrating and/or purifying biological samples. The heart of the Sample Trap is a fused silica capillary column. Available prepackaged capillary columns include: reversed-phase C18, high-carbon load, 5µm/300Ä spherical silica, and an SCX (Strong Cation Exchange) 5µm/85A material. Each column supports a recommended maximum sample loading capacity of approximately 0.1 µg with a capillary bed volume at 0.19µL or less. Unpacked and Conductive Column Assemblies are also available.
Capillary Sample Trap Column Assemblies include one or more 1 µm NanoFilter™ Capsules, each containing either a stainless steel (SST) or biocompatible titanium (Ti) frit. The swept volume of these unique filters is only 10nL each. Connect your 360µm OD capillary tubing directly to our Sample Trap Column Assemblies using the fittings provided. Each Capillary Sample Trap Column Assembly is rated to 5,000 psi (345 bar). The maximum flow rate for these products is 10µL/min; 250nL is the optimal flow rate.
The Conductive Sample Trap Columns allow voltage to be applied to the flow stream via stainless steel filter holders and NanoFilter Capsules. Apply voltage easily using our Insulating Mounting Bracket. These products are perfect for integration into CE or mass spectrometry systems.
Try our Column Coupler, which makes it possible to directly connect two or more columns in sequence. Please contact us for more information on our Capillary Sample Trap Columns.

SAMPLE TRAP COLUMN ASSEMBLY COMPONENTS CHART Please refer to the drawing and part number below when ordering replacement components
Column Assembly Nuts (a) Ferrules (b) Filter Holder Color (c) NanoFilter (d) Column (e) Capsule Union or Nano Filter (f)
Non-Conductive
C-1200 P-416 F-152 Tan M-125 (SST) C-1250 (C18) M-124NF (no frit)
C-1300 P-416BLK F-152 Tan M-126 (Ti) C-1250 (C18) M-124NF (no frit)
C-1500 P-416 F-152 Tan M-125 (SST) FS-1000-25 (unpacked) M-125 (SST)
C-1600 P-416BLK F-152 Tan M-126 (Ti) FS-1000-25 (unpacked) M-126 (Ti)
All nuts, ferrules, and column holders are made of PEEK™ polymer. Filter holders are made of PEEK polymer (non-conductive) or stainless steel (conductive). NanoFilter Capsule bodies are made of PEEK polymer (non-conductive) or stainless steel and PEEK (conductive) Abbreviation Key: SST = stainless steel; Ti = titanium; SCX = Strong Cation Exchange

EXAMPLE SEPERATION CHROMATOGRAM OF FIVE ANGIOTENSINS
Chromatographic Conditions, using C18 Sample Trap Column:
Binary Gradient A: Water; B: Acetonitrile w/0.1% Formic Acid
Gradient Profile 2% B for 0.1 minutes Ramp to 95% B over 10 minutes Hold at 95% B for 4minutes return to 2% B over 1 minute
Flow Rate 250nL/minute
Injected Amount = 500 femtomoles per peptide

INSULATING MOUNTING BRACKET
Insulating Mounting Bracket, shown with lead wire and Conductive Micro Tight Union , not included.
Use our new Insulating Mounting Bracket to easily integrate the following
Upchurch Scientific® products into your system or lab:
Conductive Micro Unions / Conductive Mini MicroFilter / Conductive Capillary Sample Trap Column

Just snap the product into place on the bracket. Voltage from your lead wire is conducted through the attaching stainless steel nut and screw (included), then onto the mounted product via the stainless steel clip.
The bracket's base includes two holes (#2 screw clearance) for easy mounting onto any lab surface. Dimensions are 1.25" L x.45" W x.63" H.

 
CAPILLARY SAMPLE TRAP COLUMNS
Artkelnummer Beschreibung  
Assemblies, Non-Conductive (1)
C-1200 C18 Column, SST Frit Includes (1) 2-pk C-1250 Columns and (1) M-125 NanoFilter  
C-1300 C18 Column Ti Frit Includes (1) 2-pk C-1250 Columns and (1) M-126 NanoFilter  
C-1500 Unpacked Column, SST Frits Includes (1) FS-1000-25 Column and (1) 2-pk M-125 NanoFilters  
C-1600 Unpacked Column, Ti Frits Includes (1) FS-1000-25 Column and (1) 2-pk M-126 NanoFilters  
Column Coupler and Replacement Parts (1)
Artikelnummer Beschreibung Swept Volume Menge  
C-1210 Column Coupler, PEEK - ea.  
C-1250 C18 Columns, 100µm ID x 2,5cm x 360µm OD - 2-pk  
F-152 MicroFerrule for 360µm OD tubing, PEEK - ea.  
FS-1000-25 Unpacked Column, 100µm ID x 2,5cm x 360µm OD - ea.  
M-124NF Capsule Union, no Frit, PEEK 9.5nL ea.  
M-125 1µm NanoFilter Capsules, with SST Frits 10nL 2-pk  
M-126 1µm NanoFilter Capsules, with Ti Frits 10nL 2-pk  
M-128 1µm Conductive NanoFilter Capsules, SST Frits 10nL 2-pk  
M-128NF Conductive Capsule Union, no Frit, SST 13nL ea.  
P-416 Female Nut, Natural PEEK - ea.  
P-416BLK Female Nut, Black PEEK - ea.  
P-416G Female Nut, Green PEEK - ea.  
INSULATING MOUNTING BRACKET
Artikelnummer Beschreibung  
M-447 insulating Mounting Bracket  
(1) Please refer to the Components Chart for a full list of replacement parts for each assembly. SST=Stainless Steel, Ti = Titanium

NANOTIGHTTM FITTINGS AND SLEEVES
- For Connecting Capillary Tubing to Standard 10-32 Coned Ports / 10-32 Fittings and 1/16" OD Sleeves for Capillary Tubing Sizes from 70pm - lmm OD
Upchurch Scientific® NanoTight Fittings and Sleeves are designed to connect 70µm - 1 mm OD capillary tubing to any standard 10-32 coned port normally intended for 1/16" OD tubing. Select the sleeves appropriate for the OD of your capillary tubing. For instance, choose F-239x for 190µm OD tubing, F-242x for 360µm OD tubing, and F-247x for 1/32" OD tubing. Each sleeve is color-coded for easy identification.
The 1.6" long Teflon® FEP NanoTight Sleeves withstand up to 50°C, and can be finger tightened to hold to 4,000 psi (276 bar) when using our NanoTight Fittings. For added convenience, try the NanoTight Kits, which include four F-330N NanoTight Fittings and ten NanoTight Tubing Sleeves.
Select from our expanded line of PEEK NanoTight Fittings, featuring several head style and length options. Each 10-pack of nuts includes ten Tefzell F-142N Ferrules.

NANOTIGHT FITTINGS
All NanoTight Nut 10-packs include a 10-pack of Ferrules (F-142Nx)
Artikelnummer Bescheibung Bild Menge  
F-142Nx Ferrules, Natural Tefzel (ETFE) 10-pk  
F-330N Long Nuts, Std. Head, Natural PEEK, 10-32 ea. Mindestabnahme 25 Stück  
F-331N Short Nuts, Std. Head, Natural PEEK, 10-32 ea. Mindestabnahme 25 Stück  
F-332N Short Nuts, Hex Head, Natural PEEK, 10-32 ea. Mindestabnahme 25 Stück  
F-333Nx Short Nuts, Headless, Natural PEEK, 10-32 10-pk  
F-334N Long Nuts, Knurl/Hex Fat Head, Natural PEEK, 10-32 ea. Mindestabnahme 25 Stück  
F-335N Long Nuts, Hex Head, Natural PEEK, 10-32 ea. Mindestabnahme 25 Stück  
F-336N Long Nuts, Headless, Natural PEEK, 10-32 ea. Mindestabnahme 25 Stück  

NANOTIGHT FEP TUBING SLEEVES, 1/16" OD
Artikelnummer ID For Tubing OD Size Color Menge  
F-237 125µm (.005") 70 - 110µm Red ea.  
F-238 180µm (.007") 125-165µm Yellow ea.  
F-239 215µm (.0085") 160-200µm Natural ea.  
F-240 280µm(.011") 225 - 265µm Blue ea.  
F-241 330µm (.013") 275-315µm Orange ea.  
F-242 395µm (.0155") 340-380µm green ea.  
F-243 455µm (.018") 400-440µm Black ea.  
F-244 535µm (.021") 480-520µm Natural ea.  
F-245 610µm (.024") 555-595µm Red ea.  
F-246 685µm (.027") 630-670µm Yellow ea.  
F-247 840µm (.033") 785-825µm Green ea.  
F-252 1.07mm (.042") 1mm Purple ea.  
NANOTIGHT KITS
Artikelnummer Beschreibung  
1238 F-238x Tubing Sleeves and Fittings Kit  
1239 F-239x Tubing Sleeves and Fittings Kit  
1240 F-240x Tubing Sleeves and Fittings Kit  
1241 F-241x Tubing Sleeves and Fittings Kit  
1242 F-242x Tubing Sleeves and Fittings Kit  
1243 F-243x Tubing Sleeves and Fittings Kit  
1244 F-244x Tubing Sleeves and Fittings Kit  
1245 F-245x Tubing Sleeves and Fittings Kit  
1246 F-246x Tubing Sleeves and Fittings Kit  
1247 F-247x Tubing Sleeves and Fittings Kit  
1252 F-252x Tubing Sleeves and Fittings Kit  
1250 NanoTight Connector Kit
Kit contains: (6) each of FEP Tubing Sleeves F-239 - F-247;
(5) each of F-330N and F-331 N NanoTight Fittings; (5) each of F-335N and
F-336N NanoTight Fittings; and (2) P-779-01 NanoTight Unions.
  

NANOTIGHTTM UNIONS
 NANOTIGHT Y CONNECTOR
- Only 17nL of Swept Volume
Upchurch Scientific® NanoTight Unions improve capillary tubing connections in several ways. The sleeving system and internal design of the unions greatly reduce the incidence of tubing misalignment. The webbed thru-hole minimizes breaking of fused silica at the junction point while adding only miniscule swept volume (8nL for the P-779-01 and 13nL for the U-411). The results are fewer blockages, fewer flow rate reductions and fewer back pressure problems.
Connect capillary tubing with these unions using the NanoTight Fittings and Sleeves listed on page 38. These versatile unions can also be used to connect capillary tubing to 1/16" OD tubing, or to connect two 1/16" OD tubing lines. To connect 1/16" OD tubing with our P-779-01, use any standard polymer 10-32 coned fittings, such as our NanoTight Fittings. To connect 1/16" OD tubing with our U-411, use the included fittings.
The pressure holding capability of the NanoTight Unions depends on the fittings and tubing sleeves selected. Please refer to the pressure ratings of these products.		 This product improves laminar flow over traditional MicroTee designs. The result is less turbulence and mixing when combining two fluid paths into one, or splitting one fluid path into two. This assembly holds to 4,000 psi (276 bar), has a .006" thru-hole, and a swept volume of only 17nL.
The Upchurch Scientific NanoTight Y Connector uses an included 1.6" long Dual-Lumen Silica NanoTight Tubing Sleeve to accommodate two 360µm OD capillary tubing lines. The single inlet/outlet end of the Y Connector uses the included F-242 NanoTight Tubing Sleeve, also designed for 360µm OD capillary tubing.
The NanoTight Y Connector comes complete with NanoTight Fittings. The body of this product is made of PEEK™ polymer, while both of the included tubing sleeves are made of Teflon® FEP. Replacement NanoTight Tubing Sleeves and fittings are available on page 38. Use F-242 replacement sleeves or choose from the other available sleeves to connect capillary tubing with ODs ranging from 70µm to 1 mm. Dual-Lumen F-235 Sleeves are listed below, and are only available for 360µm OD tubing.

NANOTIGHT UNIONS
Artikelnummer Beschreibung Threads Thru-hole Swept Volume  
P-779-01 Union, PEEK, Natural 10-32 to 10-32 .005" (130µm) 8nL  
U-411 Union, Stainless Steel Includes (2) U-400 and (2) 10-32 to 10-32 .007" (180µm) 13nL  

MICRO STATIC MIXING TEE CONDUCTIVE PERFLUOROELASTOMER FERRULE
- For Mass Spec Electrospray Applications 
The Upchurch Scientific® Micro Static Mixing Tee has a low swept volume of only .95µL and is designed for flow rates of 20-250µL/min. The center port features a 0.5µm PEEK™ polymer frit to aid in mixing. This frit adds a maximum of 20 psi (1.4 bar) back pressure to most systems (within the stated flow rate range). Constructed of PEEK and Kel-F® (PCTFE), this mixing tee is chemically resistant and biocompatible. It handles a maximum pressure of 5,000 psi (345 bar). Connect capillary tubing using our NanoTight™ Sleeves. No sleeves are required for 1/16" OD tubing.
 Try the Upchurch Scientific Conductive Perfluoroelastomer Ferrule in your electrospray applications. Unlike most graphite ferrules, the elastomeric properties of this ferrule let you use it through many tightening/ retightening cycles. It also eliminates any possibility of graphite contamination in your system. The robust qualities of perfluoroelastomer allow the ferrule to be used at temperatures from -15°C to 260°C.
Connect 360µm OD fused silica or PEEK polymer tubing into any standard 10-32 coned port designed for 1/16" OD tubing with this ferrule. Like graphite ferrules, you can apply voltage through a metallic port block or metallic nut, allowing voltage to translate to the flow path through the ferrule.
Use the Conductive Perfluoroelastomer Ferrule with any of the Fingertight Nuts listed on page 14 or NanoTight™ Nuts. To order one of these nuts without its standard ferrule, replace the "x" at the end of the nut's product number with "-01 ". Please Note: the "-01 " denotes a single nut without the accompanying ferrule.


MICRO STATIC MIXING TEE
Artikelnummer Beschreibung Thru-hole Swept Volume  
M-540 Micro Static Mixing Tee'; for 1/16" OD tubing Includes (3) F- 132 and (3) P-416 Fittings .010" (25mm) .95µL (1)  
CONDUCTIVE PERFLUOROELASTOMER FERRULE
Artikelnummer Beschreibung Menge  
M-215 Conductive Perfluoroelastomer Ferrule, Black For 360µm OD Tubing ea.  
(1) Includes fret volume
(2) The frit in this product cannot be changed,

1/16" OD PEEK™ TUBING SLEEVES
-For Connecting Capillary Tubing to Standard 10-32 Ports / Require the Use of Wrench Tightened Stainless Steel Nuts
Like the NanoTight™ FEP Sleeves, these PEEK Tubing Sleeves are designed to be used with 1/16" OD, 10-32 threaded fittings to adapt capillary tubing to standard coned ports. Made of PEEK polymer, these 1.3" long sleeves can be used up to 125°C and will hold to 6,000 psi (414 bar).
Please Note: These sleeves require a wrench tightened stainless steel nut to achieve proper sealing. We recommend our F-140 Two-Piece Fingertight Fitting, which includes a PEEK ferrule. Many researchers also use a stainless steel nut and ferrule with these sleeves, such as our U-400 and U-401. For a less expensive alternative that doesn't require wrench tightening, consider our NanoTight Tubing Sleeves and Kits.
1/32" OD TEFLON® FEP TUBING SLEEVES
These 1.6" long sleeves facilitate connecting capillary tubing into ports designed for 1/32" OD tubing. Please refer to the product listing below to select the appropriate sleeve for your capillary OD size. For instance, choose F-376 for 360µm OD and F-373 for 190µm OD capillary tubing. These sleeves are pressure rated to 1,750 psi (121 bar) and can be used at up to 50°C.		 1/32" OD PEEK TUBING SLEEVES
Upchurch Scientific® 1/32" OD PEEK Tubing Sleeves are specifically designed to connect capillary tubing to our Nano Flow Sensor and 6-32 NanoPeak™ Valves. The 1.6" long sleeves can also be used with any fitting designed for 1/32" OD tubing when smaller tubing must be connected. Select the appropriate sleeve from the product listing for your capillary tubing OD size. The 1/32" OD PEEK Tubing Sleeves have a pressure rating of 5,000 psi (345 bar) and a maximum recommended temperature of 125° C.

1/16" OD PEEK TUBING SLEEVES
Artikelnummer ID For Tubing OD Size Color Menge  
F-225 125µm (.005") 70 - 110µm red ea.  
F-226 180µm (.007") 125-165µm Yellow ea.  
F-227 230µm (.009") 175-215µm Yellow ea.  
F-228 250µm (.011") 225-265µm Blue ea.  
F-229 330µm (.013") 275-315µm Natural ea.  
F-230 405µm (.016") 350-390µm Orange ea.  
F-231 560µm (.022") 505-545µm natural ea.  
F-234 885µm (.027") 630-670µm Yellow ea.  
F-232 785µm (.031") 730-770µm Natural ea.  
F-233 865µm (.034") 785-825µm Blue ea.  
1/32" OD PEEK TUBING SLEEVES
F-380 125µm (.005") 70-110µm Red ea.  
F-381 180µm (.007") 125- 765µm Yellow ea.  
F-382 205µm (.008") 150-190µm Natural ea.  
F-383 230µm (.009") 175-215µm Gray ea.  
F-384 255µm (.010") 200-240µm Blue ea.  
F-387 250µm (.011") 225-265µm red ea. Mindestabnahme 25 Stück  
F-388 330µm (.013") 275-315µm Black ea.  
F-385 380µm (.015") 325-365µm Natural ea.  
F-386 510µm (.020") 455-495µm Orange ea.  
1/32" OD TEFLON FEP TUBING SLEEVES
F-370 75µm (.003") 20 -60µm Natural ea.  
F-371 125µm (.005") 70-110µm red ea.  
F-372 180µm (.007") 125 - 165µm Yellow ea.  
F-373 230µm (.009") 175-215µm Natural ea.  
F-374 280µm (.011") 225-265µm Blue ea.  
F-375 330µm (.013") 275-315µm Orange ea.  
F-376 395µm (.0155") 340-380µm Green ea.  

RHEFLEX® M4 FITTINGS
For Coned Ports
For 1/32" OD Tubing		 VICI® (VALCO)-COMPATIBLE FITTINGS, 6-40 THREADS
For Coned Ports
For 1/32" OD and Capillary Tubing
The Rheodyne® RheFlex M4 Fitting is designed to connect 1/32" OD tubing in MX Module applications. This PEEK™ fitting has a one piece design, which eliminates the need for a separate nut and ferrule. The M4 Fitting design provides dependable zero dead volume connections for micro and nano applications. Due to the unique RheFlex gripping design, the M4 Fitting will hold to 5,000 psi (345 bar) on PEEK or fused silica tubing. A PEEK M4 Plug is also available. Use Rheodyne ChromTRAC™ knobs with the RheFlex M4 Fittting for fingertight convenience and to color-code connections. The Upchurch Scientific™ M-645 Fitting for 1/32" OD tubing is a robust, one-piece alternative for the Valco Nanovolume™ 6-40 threaded fitting. Our product consists of a PEEK polymer nut and an integrated Kel-F® (PCTFE) ferrule. Capillary tubing can also be connected with this fitting using the 1/32" OD Teflon® Tubing Sleeves. Please refer to the table below for pressure rating data. Also available is the P-552 Plug, for unused 6-40 threaded Valco ports.

PRESSURE RATINGS
Fitting for Valco-Compatoble 6-40 Port M-645 1/32" OD Tubing Sleeves Tubing Pressure rating
- FEP, 1/32" OD x.005" ID 3,000psi (207bar)
- PEEK, 1/32" OD x.005" ID 2,750psi (190 bar)
- Stainless Steel, 1 /32" OD x.005" ID 3,250psi (224 bar)
F-376, 395µm ID FEP, 360µm OD x 75µm ID 1,750psi (121 bar)
F-376, 395µm ID PEEK, 360µm OD x 75µm ID 1,750psi (121 bar)
F-376, 395µm ID Fused Silica, 360µm OD x 75µm ID 1,750psi (121 bar)
 This chart is intended for reference only and refers to the pressure rating of the fittings. NOT the tubing. Tests were conducted with room temperature water as the solvent. Your results may vary depending on the specific port and tubing materials tubing ID, actual tubing diameters (with manufacturers' tolerances), temperature, chemical compatibility, etc. The fittings were finger tightened for these tests.

Artikelnummer Bild Beschreibung Menge  
RHEFLEX FITTINGS FOR CONED PORTS
6000-360 M4 RheFlex Fittings, for 1/32" OD Tubing, Natural PEEK 10-pk  
VICI (VALCO)-COMPATIBLE FITTINGS FOR 6-40 CONED PORTS
M-645 Fittings for 1/32" DD Tubing, Natural PEEK/Kel-F ea.  
P-552 Plug for 1/32" OD Tubing Port, Natural PEEK/Kel-F ea.  

LEE COMPANY "MINSTAC®" COMPATIBLE FITTINGS, 6-40 THREADS For MINSTAC Valve Ports
- Easy to Use / For 1/16" Ob and Capillary Tubing		 SUPER FLANGELESS FITTINGS, 6-40 AND 6-32 THREADS For Flat-Bottom Ports
- For 1/16" OD and Capillary Tubing
Upchurch Scientific® TinyTight™ Fittings are easy-to-use alternatives for Lee Company 062 MINSTAC fittings systems. These Upchurch Scientific fittings consist of a 6-40 threaded PEEK™ polymer nut and a TinyTight Ferrule. To use, simply slide a fitting head-first onto your tubing, followed by the ring and ferrule, and thread this assembly into the solenoid valve receiving port, while making sure the tubing is bottomed out. No collets, colleting tools or chamfering tools required!
Choose from two ferrule options, with .020" and .030" thru-holes. Both are designed to connect 1/16" OD tubing. You may also connect capillary tubing having outside diameters from 70µm to 1 mm with these ferrules, using our NanoTight™ Sleeves.		 This Upchurch Scientific fittings system connects 1/16" OD and capillary tubing into any flat-bottom 6-40 or 6-32 threaded port. The 316 stainless steel ring of the Super Flangeless system offers constant, uniform compression on the ferrule while operating as a bearing against the nut. Thus, the nut can spin freely against the ferrule system, preventing the tubing from twisting during the tightening process. Once swaged, the lock ring holds the ferrule in place on the tubing, a benefit that also makes this fittings style ideal for tubing assemblies since the ferrule keeps the nut from sliding off the tubing.
Connect 1/16" OD tubing into 6-40 flat-bottom ports using the M-650 Ferrule and M-644-03 Nut. For 6-32 flat-bottom ports, use the M-650 Ferrule with the M-660 Nut. Use our Teflon® FEP NanoTight Sleeves with these fittings to connect capillary tubing with OD sizes between 70µm and 1 mm. Both headless nuts are made of PEEK polymer. For easy tightening, try our N-290 Extender Tool on.

TINYTIGHT SWAGING TOOL
Make quick and easy 6-40 TinyTight tubing assemblies with this new tool. The tool is specifically designed for use with the Upchurch Scientific M-647 and M-657 Ferrules, 6-40 threaded M-644-03 Nut and 1/16" OD tubing. To use, first place the tool in a vise, then tighten tubing, fitting and ferrule into the tool as you would into any port. Once removed, the swaged ferrule will be held in place on the tubing.

PRESSURE RATINGS
  1/16" OD Tubing Sleeves Tubing Pressure Ratings
Fittings for MINSTAC•
Compatible 6-40 Port
M-647 / M-657 M-644-03
 - - - FEP,1/16"ODx.010"ID
PEEK, 1/16" OD x.010" ID
Stainless Steel, 1/16" OD x.010" ID		 600 psi (41 bar)
1,500 psi (103 bar)
4,000 psi (276 bar)
Fittings for 6-40 Port
M-650, M-644-03
 - - - F-376, 395µm ID FEP,1/16"ODx.010"ID
PEEK, 1/16" OD x.010" ID
Stainless Steel, 1/16" Of x.010" ID
Fused Silica, 360µm OD x 75µm ID		 1,750 psi (121 bar)
3,750 psi (259 bar)
3,750 psi (259 bar)
750 psi (52 bar)
Fittings for 6-32 Port
M-650, M-660
 - - - F-376, 395µm ID FEP, 1/16" OD x.010" ID
PEEK, 1/16" OD x.010" ID
Stainless Steel, 1/16" OD x.010" ID
Fused Silica, 360µm OD x 75µm ID		 1,750 psi (121 bar)
3,750 psi (259 bar)
3,750 psi (259 bar)
750 psi (52 bar)
This chart is intended for reference only and refers to the pressure rating of the fittings, NOT the tubing. Tests were conducted with room temperature water as the solvent. Your results may vary depending on the specific port and tubing materials, tubing ID, actual tubing diameters (within manufactures´tolerances), temperature, chemical compatibility, etc. The fittings were finger tightened for these tests

Artikelnummer Beschreibung Bild Menge  
LEE COMPANY-COMPATIBLE FITTINGS FOR 6-40 PORTS
M-644-03 Headless Nuts for 1/16" OD tubing, Green PEEK ea.  
M-647 TinyTight Ferrules for 1/16" OD tubing,
.020" thru-hole, Natural PEEK/SST		 ea.  
M-657 TinyTight Ferrules for 1/16" OD tubing,
.030" thru-hole, Black PEEK/SST		 ea.  
SUPER FLANGELESS FITTINGS FOR 6-40 AND 6-32 FB PORTS
M-644-03 Headless Nuts for 1/16" OD tubing, 6-40, Green PEEK    ea.  
M-650x Ferrules for 1/16" OD tubing, Natural PEEK/SST    10-pk  
M-650 Headless Nuts for 1/16" OD tubing, 6-32, Natural PEEK ea.  
TINYTIGHT SWAGING TOOL
M-150 6-40 Tiny'right Swaging Tool, SST    
FB = Flat-Bottom; SST = Stainless Steel